Effect of 2-Hydroxy-4-methoxy Benzoic Acid Isolated from Hemidesmus indicus on Erythrocyte Membrane Bound Enzymes and Antioxidant Status in Streptozotocin-induced Diabetic Rats
نویسندگان
چکیده
In the present study, the effect of 2-hydroxy-4-methoxy benzoic acid isolated from roots of Hemisdesmus indicus on the erythrocyte membrane bound enzymes and antioxidant status in streptozotocin-induced diabetic rats was investigated. The streptozotocin-induced diabetic rats were treated with 2-hydroxy-4-methoxy benzoic acid (500 μg/kg/day) for 7 weeks by oral intubation and compared with glibenclamide, a standard hypoglycemic agent (100 mg/kg). The erythrocyte membrane was isolated and the activity of Na(+)/K(+)-dependent ATPases, Ca(2+)-ATPases, Mg(2+)-ATPases were determined. Superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, vitamins C, vitamin E, plasma reduced glutathione and erythrocyte glutathione, reduced glutathione content in the tissues was also assayed. Administration of 2-hydroxy-4-methoxy benzoic acid to diabetic rats significantly (F>0.05 and P<0.001) elevated the activity of total ATPases, Na(+)/k(+) ATPase, Mg(2+) ATPase and Ca(2+) ATPase to near normal level. The activities of catalase, superoxide dismutase and glutathione peroxidase and glutathione-S-transferase in erythrocytes were decreased significantly (F>0.05; P<0.001) in diabetic rats. Diabetic rats treated with 2-hydroxy-4-methoxy benzoic acid showed a significant (F>0.05; <0.001) increase in the enzymic antioxidants in erythrocytes. The elevated levels of vitamin E and low level of vitamin C and glutathione level in plasma and erythrocytes were observed in diabetic rats when compared to control rats and were restored significantly (F>0.05; P<0.001) after the administration of 2-hydroxy-4-methoxy benzoic acid. This study concludes administration of 2-hydroxy-4-methoxy benzoic acid supports the restoration of antioxidant defence, reduces the free radial production, lipid peroxidation and the glycosylation of haemoglobin in diabetic rats.
منابع مشابه
2-hydroxy 4-methoxy benzoic acid isolated from roots of Hemidesmus indicus ameliorates liver, kidney and pancreas injury due to streptozotocin-induced diabetes in rats.
Protective effect was evaluated in streptozoticin (STZ)-induced diabetes rats. 2-Hydroxy 4-methoxy benzoic acid (HMBA) was isolated from the roots of Hemidesmus indicus and administered (500 microg/kg body weight) orally for 7 weeks to STZ-induced diabetic and non-diabetic rats to study its effect on protein metabolism, serum electrolytes and on liver and kidney lipid peroxides. Oral administra...
متن کاملEffect of 2-hydroxy 4- methoxy benzoic acid isolated from the roots of Hemidesmus indicus on blood glucose and tissue lipid peroxidation in streptozotocin-induced diabetic rats
Effect of 2-hydroxy 4methoxy benzoic acid isolated from the roots of Hemidesmus indicus on blood glucose and tissue lipid peroxidation in streptozotocin-induced diabetic rats M. Gayathri, K. Kannabiran* *School of Biotechnology, Chemical and Biomedical Engineering,VIT University, Vellore-632 014, Tamil Nadu, India. For correspondence:Dr. K.Kannabiran,Professor, School of Biotechnology, Chemical...
متن کاملHypoglycemic activity of Hemidesmus indicus R. Br. on streptozotocin-induced diabetic rats
OBJECTIVE To evaluate the antidiabetic activity of an aqueous extract of the roots of Hemidesmus indicus on blood glucose, serum electrolytes, serum marker enzymes, liver microsomal P-450 enzymes, and lipid peroxidation in the liver and kidney of streptozotocin-induced diabetic rats. MATERIALS AND METHODS Effect of H. indicus extract on blood glucose was studied with fed, fasted and glucose-l...
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Background: Hemidesmus indicus is an important medicinal plant and extensively used in Ayurvedic and Unani system of medicine. The aim of the study was to evaluate the antioxidant, hypoglycaemic and hypolipidemic potential of methanolic extract of roots of Hemidesmus indicus (HIE) in streptozotocin (STZ) induced diabetic mice. Methods: HIE was analyzed by LC-MS to determine its phytochemical co...
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Many traditional plant treatments for diabetes mellitus are used throughout the world and few of the traditional plant treatments for diabetes have been shifted scientific scrutiny. The present study aimed to study the effect of Hemidesmus indicus plant leaf extract on the liver enzymes in alloxan induced diabetic Wister rats. Hemedesmus indicus plant was successively extracted with different o...
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عنوان ژورنال:
دوره 74 شماره
صفحات -
تاریخ انتشار 2012